Protective Effects of Interleukin-10 on Diaphragm Muscle in a Septic Animal Model
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AZUSA TANEDA, CHIYOHIKO SHINDOH, YUZURU OHUCHI and KUNIO SHIRATO
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The First Department of Internal Medicine, Tohoku University School of Medicine, Sendai 980-8574
The effects of Interleukin (IL)-10 intravenous injection after endotoxin administration on diaphragm muscle were studied using Wistar rats. The animals were divided into two treatment groups: A saline + endotoxin group as control and an IL-10 + endotoxin group. E. coli endotoxin (10 mg/kg) was injected intraperitoneally 5 minutes after saline or IL-10 (1250 U, 0.25 mg) injection. The force-frequency curves, twitch kinetics and fatigability were measured at 0 and 4 hours after endotoxin injection. In the saline + endotoxin group, the force-frequency curves and half relaxation time were significantly decreased at 4 hours (p<0.001 and p<0.05, respectively) compared to those at 0 hour. In the IL-10 + endotoxin group, the decrement in the force-frequency curves by endotoxin was prevented at 4 hours compared to that at 0 hour. Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry showed positive staining in the saline + endotoxin at 4 hours, but there was no significant staining at 0 or 4 hours in the IL-10 + endotoxin group. These data suggest that IL-10 prevents the deterioration of contraction induced by endotoxin by inhibiting nitric oxide production in the diaphragm muscle.
Key words---
endotoxin; nitric oxide; NADPH diaphorase
© 1998 Tohoku University Medical Press
Tohoku J. Exp. Med., 1998, 185, 45-54
Address for reprints:
Chiyohiko Shindoh, M.D., The First Department of Internal Medicine, Tohoku University School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai 980-8574, Japan.
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