Tohoku J. Exp. Med., 1999, 187 (2)

Identification of Truncated Human Glutamate Transporter

YASUHIRO MATSUMOTO, TAKEMI ENOMOTO and TAKASHI MASUKO

Department of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578

  • Excitatory amino-acid carrier 1 (EAAC1) is a high affinity Na+-dependent L-glutamate/D, L-Aspartate transporter protein. A truncated form of EAAC1 (tEAAC1) was identified by reverse transcription and polymerase chain reaction in the human cell line, ACHN, in which EAAC1 mRNA was highly expressed. The deduced amino acid sequence of tEAAC1 lacks 31-77 amino acids including the first extracellular domain. The mRNA encoding tEAAC1 was detected in various cells of human origin but not in cells of rat or mouse origin. The expression of tEAAC1 mRNA was proportional to that of full-length EAAC1 (fEAAC1) mRNA, suggesting common transcriptional regulation between tEAAC1 and fEAAC1. In addition, the expression of EAAC1 mRNA was relatively low or non-existent in non-adherent cells.
    Key words--- RT-PCR; EAAC1; EAAT3; glutamate transporter
    © 1999 Tohoku University Medical Press

    Tohoku J. Exp. Med., 1999, 187, 173-182
    Address for reprints: Takashi Masuko, Department of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Aoba Aramaki, Aoba-ku, Sendai 980-8578, Japan.
    e-mail: masuko@mail.pharm.tohoku.ac.jp

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