Tohoku J. Exp. Med., 1999, 187 (3)

Inhibitory Effects of Transforming Growth Factor-b1 Pretreatment on Experimental Pulmonary Metastasis of MCS-1 Chinese Hamster Mesenchymal Chondrosarcoma Cells

NOBUYOSHI FUJISAWA, NORIMITSU L. SATO and TEI-ICHI MOTOYAMA1

Institute for Laboratory Animals, Niigata University School of Medicine, Niigata 951-8510, and 1The Second Department of Pathology, Yamagata University School of Medicine, Yamagata 990-9585

  • Recent studies have suggested that transforming growth factor(TGF)-b1 acts as a multifunctional regulator of cell growth, and also modifies tumor progression and metastasis. In the present study, we investigated the effects of TGF-b1 on the proliferation and experimental pulmonary metastasis of MCS-1. MCS-1 are undifferentiated type cloned tumor cells established from a mesenchymal chondrosarcoma which spontaneously occurred in the soft tissue of a female Chinese hamster. MCS-1 cells were pretreated with TGF-b1 (0, 0.05, 0.5, 2, 10 ng/ml) for 72 hours in a medium containing 1% fetal bovine serum, then tested for in vitro growth by the MTT method, in vivo growth by subcutaneous inoculation into athymic nude mice (1´106 cells/mouse) and experimental pulmonary metastasis by injection into the lateral teil vein of athymic nude mice (5´104 cells/mouse). TGF-b1 significantly inhibited in vitro growth of MCS-1, depending on its concentrations, and also experimental metastasis with maximal inhibition at 0.5 or 2 ng/ml treatment compared to untreated controls. TGF-b1, however, was ineffective for in vivo subcutaneous growth of MCS-1. These results indicated that TGF-b1 might be an inhibitor of metastasis of mesenchymal chondrosarcomas including other types of non-epitherial cartilage or bone formation tumors.
    Key words--- experimental metastasis; TGF-b1; mesenchymal chondrosarcoma; Chinese hamster
    © 1999 Tohoku University Medical Press

    Tohoku J. Exp. Med., 1999, 187, 203-213
    Address for reprints: Nobuyoshi Fujisawa, Institute for laboratory animals, Niigata University School of Medicine, 1 Asahimachi-dori, Niigata 951-8510, Japan.
    e-mail: arinomi@med.niigata-u.ac.jp

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