Tohoku J. Exp. Med., 1999, 187 (3)

Localization of PDK-1 mRNA in the Brain of Developing and Adult Rats

SHUICHI YOSHIDA,1,2 HIROYUKI SAKAGAMI,1 YUJI OWADA,1 SHOICHI KOKUBUN2 and HISATAKE KONDO1

1Division of Histology, Department of Cell Biology, and 2Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Tohoku University, Sendai 980-8575

  • Gene expression for 3-phosphoinositide-dependent protein kinase-1 (PDK-1) in developing and adult rat brains was examined by in situ hybridization histochemistry. In embryonic days, the mRNA was evident throughout the entire neuraxis. The expression remained evident throughout the entire gray matters until postnatal day 7, and thereafter it decreased overall in the mantle and ventricular zones except for the cerebellar Purkinje and granule cell layers, the olfactory and hippocampal neuronal layers. The pattern of this gene expression is similar to those of for protein kinase B and class I phosphoinositide 3-kinases.
    Key words--- 3-phosphoinositide-dependent protein kinase-1; Rat; Brain; Development; in situ hybridization
    © 1999 Tohoku University Medical Press

    Tohoku J. Exp. Med., 1999, 187, 249-255
    Address for reprints: Shuichi Yoshida, Division of Histology, Graduate School of Medical Sciences, Tohoku University, Sendai 980-8575, Japan.
    e-mail: yoshida@gonryo.med.tohoku.ac.jp

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