Tohoku J. Exp. Med., 1999, 187 (3)

Evaluation of a New Anti-HCV-Assay Kit for Anti-HCV Screening in Early Childhood

KOHACHIRO SUGIYAMA, KENJI GOTO, YOSHISHIGE MIYAKE, FUMIYA MIZUTANI, KOJI TERABE, ROU LI, HIROKO MANIWA, YOSHIKAZU KAWABE and YOSHIRO WADA

Department of Pediatrics, Nagoya City University Medical School, Nagoya 467-8601

  • Sera of 20 children falsely identified as positive for hepatitis C virus antibody (Anti-HCV) by a second generation anti-HCV-assay kit (Imucheck-HCV Ab “Kokusai”) were re-tested using a new third generation anti-HCV-assay kit (Imucheck·F-HCV C50 Ab “Kokusai”). Seventeen of the samples were reclassified as negative and only three remained positive. Changing well solids in the anti-HCV-assay kit from casein to bovine serum albumin appears to have improved the false-positive rate, most likely as a result of decreased non-specific adsorption of casein antibodies.
    Key words--- Anti-HCV; false positive; casein antibodies
    © 1999 Tohoku University Medical Press

    Tohoku J. Exp. Med., 1999, 187, 257-262
    Address for reprints: Kohachiro Sugiyama, Department of Pediatrics, Nagoya City University Medical School, Kawasumi-cho, Mizuho-ku, Nagoya 467-8601, Japan.
    e-mail: sugiyama@med.nagoya-cu.ac.jp

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