Creatinine at the Evaluation of Urinary 1-Methyladenosine and Pseudouridine Excretion
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ICHIYO HONDA, KUNIHIKO ITOH,1 MICHINAO MIZUGAKI1 and TAKESHI SASAKI
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Department of Clinical and Laboratory Medicine, Tohoku University School of Medicine, and 1Department of Pharmaceutical Sciences, Tohoku University Hospital, Sendai 980-8574
The elevation of urinary modified nucleosides levels in urine is found in patients with cancers. In the precent study, we have tested 616 urine samples randomly collected from non-malignant cases. Thirty-two percent (194/616) and 11% (68/616) had elevated levels of 1-methyladenosine and pseudouridine, respectively (They are designated as false-positive cases). To elucidate the cause on non-specific elevation of the nucleosides, the correlation between creatinine excretion level and urinary nucleosides levels were determined. The result revealed that false-positive cases were frequently detected in patients with lower creatinine excretion levels. The mean creatinine levels of false-positive cases were significantly lower than those of negative cases. From these results, the false-positive of urinary 1-methyladenosine and pseudouridine might be due to the low creatinine excretion mainly caused by the renal dysfunction. Creatinine excretion in each individual should be taken into consideration in case of determining urinary modified nucleosides.
Key words---
modified nucleosides; 1-methyladenosine; pseudouridine; creatinine excretion
© 1999 Tohoku University Medical Press
Tohoku J. Exp. Med., 1999, 188, 133-138
Address for reprints:
Address for reprints: Ichiyo Honda, Department of Internal Medicine, National Iwate Hospital, 48 Dorotayamashita, Yamame, Ichinoseki, Iwate 021-0015, Japan.
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