Regular Contributions
Four New Human Renal Cell Carcinoma
Cell Lines Expressing Globo-Series Gangliosides
- MAKOTO SATOH,
FATEMEH MOOSAVI
NEJAD, OSAMU NAKANO,
AKIHIRO ITO, SADAFUMI
KAWAMURA, CHIKARA
OHYAMA, SEIICHI SAITO
and SEIICHI ORIKASA
Department of Urology, Tohoku University
School of Medicine, Sendai 980-8574
Clinicopathological studies
revealed that monosialosyl galactosyl globoside (MSGG) and disialosyl
galactosyl globoside (DSGG) expressed by renal cell carcinoma (RCC)
are one of the biochemical indicator related to the metastatic
potential. The present study examines the characteristics of four new
human RCC cell lines and compares the expression of MSGG and DSGG
among them using TLC immunostaining and flow cytometry. TOS-1 and
TOS-2 were derived from metastatic subcutaneous tissues. TOS-3 and
TOS-3LN were derived from the primary lesion and from metastatic
lymph nodes respectively. Monolayer culture, light microscopy and
electron microscopy of these cells showed that these cell lines were
derived from RCC. TLC immunostaining and flow cytometric analysis
revealed increased levels of MSGG in TOS-2 and TOS-3LN, and increased
DSGG in TOS-1 and TOS-3LN. These cell lines would be useful for
functional studies of globo-series ganglioside expressed by RCC.
Key words--- renal cell carcinoma;
established cell lines; globo-series
ganglioside
© 1999 Tohoku University Medical Press
Tohoku J. Exp. Med., 1999,
189,95-105
Address for reprints: Makoto Satoh, M.D., Department of
Urology, Tohoku University School of Medicine, 1-1 Seiryomachi,
Aoba-ku, Sendai 980-8574, Japan.
e-mail: msatoh@uro.med.tohoku.ac.jp
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