Regular Contributions
The Expression of Common Fragile
Sites in Peripheral Blood Lymphocytes of Breast and Colorectal Cancer
Patients with Aphidicolin.
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AYSÅúE
BALCI, ABDULLAH
EKMEKCİ and RECEP
ÇETİN1
Department of Medical Biology and
Genetics, Faculty of Medicine, Gazi University, Ankara, and
1Department of Surgery, Faculty of Medicine,
Süleyman Demirel University, Isparta, Turkey
The frequency and
distribution of aphidicolin induced common fragile sites was
evaluated on chromosomes of peripheral blood lymphocytes in 10 breast
and 10 colorectal cancer patients, and 10 healthy controls to
determine correlation between specific fragile sites and cancer
breakpoints. Fifty complete metaphases were screened from each
culture and the results were evaluated by Student's t-test.
The total number of fragile sites was found as 933 in breast cancer
patients, 950 in colorectal cancer patients and 501 in control group.
Both the number of aberrations per cell and number of aberrations per
damaged cell were significantly higher in the patient groups. These
findings indicate that genetic instability in the breast and
colorectal cancer patients increased and fragile sites may play a
critical role in the pathogenesis of breast and colorectal
cancer.
Key words--- fragile sites; colorectal
cancer; breast cancer and aphidicolin
© 1999 Tohoku University Medical Press
Tohoku J. Exp. Med., 1999,
189, 107-116
Address for reprints: Abdullah Ekmekci, Ph.D., Department of
Medical Biology and Genetics, Faculty of Medicine, Gazi University,
06510 BesÅúevler, Ankara, Turkey.
e-mail: ergun@tr-net.net.tr
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