Comparative Studies on the Cysteine Proteinase Inhibitory Capacity of Mammalian Blood
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AKIO SAITO and YASUMORI KITANO
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Department of Biochemistry, School of Medicine, Kinki University, Osaka-Sayama 589-8511
The inhibition of cysteine proteinase (papain) by human, bovine, horse, rabbit, guinea pig, rat, hamster, and mouse plasma, and pig, sheep, goat, and dog sera was investigated. The rat and mouse plasma and the pig serum showed such high inhibitory capacity as 813, 380, and 508%, respectively, of the human plasma. On the other hand, the horse, guinea pig, and rabbit plasma indicated 36, 40, and 54%, respectively, of the human plasma. A cysteine proteinase inhibitor (s) was separated from a-macroglobulin by Sephacryl S-300, and the inhibitory activity of all of the mammalian plasma/sera except for the guinea pig plasma appeared at around the 50-150-kDa region. The guinea pig plasma exhibited well-resolved two peaks of 100 and 200 kDa.
Key words---
cysteine proteinase inhibitor; mammalian plasma/serum; papain inhibitory capacity
© 2000 Tohoku University Medical Press
Tohoku J. Exp. Med., 2000, 190, 83-92
Address for reprints: Akio Saito, Department of Biochemistry, School of Medicine, Kinki University, 377-2 Ohno-Higashi, Osaka-Sayama 589-8511, Japan.
e-mail: asaito@med.kindai.ac.jp
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