Tohoku J. Exp. Med., 2000, 191 (3)

Rhodostomin Inhibits the Transforming Growth Factor-b1-Enhanced Adhesion Activity of ROS 17/2.8 Osteosarcoma Cells

RONG-SEN YANG and TUR-FU HUANG1

Departments of Orthopaedics, and 1Pharmacology, College of Medicine, National Taiwan University, Taipei, Taiwan

  • We have investigated the effect of transforming growth factor-b1 (TGF-b1) on the in vitro adhesion activity of the rat osteosarcoma cell lines (ROS 17/2.8) to extracellular matrix substrata, including fibronectin, type I and IV collagen, as well as laminin. The interaction of Arg-Gly-Asp (RGD) and rhodostomin, an RGD containing snake venom, with TGF-b1 on the cell adhesion was also evaluated. The results showed that incubation with various concentration of TGF-b1 (1-15 ng/ml) significantly increased the adhesion activity (1.4 to 2.5 folds) of ROS 17/2.8 to fibronectin and type I collagen (p<0.01), whereas the adhesion activity to laminin and type IV collagen was slightly elevated (1.1 to 1.5 folds). The peak effect of TGF-b1 on the cell adhesion occurred after pretreatment of ROS 17/2.8 with TGF-b1 for 6 hours. Treatment with Arg-Gly-Asp-Ser (RGDS) and rhodostomin effectively suppressed the TGF-b1-enhanced adhesion activity to fibronectin and type I collagen. This study demonstrated that the up-regulated cell adhesion activity of ROS 17/2.8 cells by the TGF-b1 can be inhibited by the rhodostomin.
    Key words--- osteosarcoma; cell adhesion; metastasis; TGF-b1; rhodostomin
    © 2000 Tohoku University Medical Press

    Tohoku J. Exp. Med., 2000, 191, 145-155
    Address for reprints: Yang Rong-Sen, M.D., Ph.D., Prof., Department of Orthopaedics, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei, Taiwan
    e-mail: Yang@ha.mc.ntu.edu.tw

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